Hematological indicators of the marsh frog during stimulated spawning
https://doi.org/10.26897/2949-4710-2024-2-2-69-74
Abstract
When breeding frogs on a farm, it is necessary to monitor their health status, and the most convenient method of control is a blood test. Hematological parameters of marsh frogs were studied before and after spawning. After hibernation at a temperature of 4°C from the first week in the pool, the frogs were subjected to hormonal stimulation with a mixture of drugs “Gonadotropin chorionic” and “Surfagon”, the next day successful spawning with 100% fertilization of eggs was observed. A reduced number of red blood cells was found in the blood of frogs after spawning. The proportion of neutrophils and eosinophils in the leukogram of frogs after spawning decreased, the number of monocytes increased 3 times, the proportion of basophils increased. The stimulated spawning of the marsh frog was reproduced for the first time in captivity under the control of an analysis of hematological parameters, which showed the possibility of using this process in raniculture conditions.
Keywords
About the Authors
R. V. ZhelankinRussian Federation
Roman V. Zhelankin, Junior Research Associate, Laboratory of Cognitive Processes and Mathematical Psychology
13 Yaroslavskaya St., Moscow, 129366
G. I. Pronina
Russian Federation
Galina I. Pronina, DSc (Bio), Associate Professor, Professor at the Department
of Aquaculture and Beekeeping
49 Timiryazevskaya St., Moscow, 127434
References
1. Van Kh.D., Mukatova M.D., Skolkov S.A. On possibility of use of lake frog (Rana ridibunda) as a raw material. Vestnik of Astrakhan State Technical University. Series: Fishing Industry. 2013;1:190-193. (In Russ.)
2. Mineeva O.V., Mineev A.K. Features of hematological parameters of lake frog Rana ridibunda Pallas, 1771 of the Saratov reservoir. Samarskaya Luka: problemy regional’noy i global’noy ekologii. 2014;23(2):178-184. (In Russ.)
3. Uteshev V.K., Shishova N.V., Kaurova S.A., Manokhin А.А., Gakhova E.N. Collection and cryopreservation of hormonally induced sperm of pool frog (Pelophylax lessonae). Russian Journal of Herpetology. 2013;20(2):105-109.
4. Kouba A.J., del Barco-Trillo J., Vance C.K., Milam C., Carr M.A. Comparison of human chorionic gonadotropin and luteinizing hormone releasing hormone on the induction of spermiation and amplexus in the American toad (Anaxyrus americanus). Reproductive Biology and Endocrinology. 2012;10:59. https://doi.org/10.1186/1477-7827-10-59
5. Meesawat S., Kitana N., Kitana J. Hematology of Wild Caught Hoplobatrachus rugulosus in Northern Thailand. Asian Herpetological Research 2016, 7(2): 131-138. https://doi.org/10.16373/j.cnki.ahr.150037
6. Romanova E.B., Nikolaev V.Yu. Immunophysiological characteristics of the populations of green frogs in urban territory. Izvestiya Samarskogo nauchnogo tsentra RAN. 2014;16(5-1):616-622. (In Russ.)
7. Zhelankin R.V. Changes in morphological and physiological parameters of adult individuals of the lake frog at different phases of technology of their feeding and breeding. Fish Breeding and Fisheries. 2021;10(189):44-56. (In Russ.) https://doi.org/10.33920/sel-09-2110-04
8. Prisny A.A., Kulko S.V., Pigaleva T.A. Influence of a constant magnetic field on morphophisyology parameters of blood system and sperm cells of Rana Ridibunda Pall. Nauchnye vedomosti Belgorodskogo gosudarstvennogo universiteta. Seriya: Estestvennye nauki. 2011;3(98):141-144. (In Russ.)
9. Arikan H., Çiçek K. Haematology of amphibians and reptiles: a review. Journal of Zoology. 2014;10(1):190-209. https://biozoojournals.ro/nwjz/content/v10n1/nwjz_143501_Cicek.pdf
10. Ivanov A.A., Pronina G.I., Koryagina N.Yu. Haematology of poikilothermic hydrobionts: monograph. Irkutsk, Russia: OOO “Megaprint”, 2018:133. (In Russ.)
11. Peskova T.Yu., Bachevskaya O.N., Plotnikov G.K. Hematological Indices of the Lake Frog Pelophylax ridibundus (Pallas, 1771) (Ranidae, Anura) Inhabiting Reservoirs in the Northwestern Ciscaucasia with Various Pollution Types. Current Studies in Herpetology. 2019;19(1-2):40-45. https://doi.org/10.18500/1814-6090-2019-19-1-2-40-45
12. Mikryakov V.R., Mikryakov D.V. Immunological indication of fish health. Journal of Ichthyology. 2015;55:(1):119-123. (In Russ.) https://doi.org/10.7868/S0042875215010129
13. Shapovalova K.V. Adaptive reactions of bone marrow and development of oxidative stress in pond and lake frogs living in different hydrochemical conditions of the environment: CSc (Bio) thesis abstract: 03.02.08. Nizhny Novgorod, Russia: Lobachevsky State University of Nizhni Novgorod, 2020:23. (In Russ.)
14. Pronina G.I., Koloskova O.V. Practicum on pathological physiology of animals: textbook. Moscow, Russia: Lan’, 2021:336. (In Russ.)
Review
For citations:
Zhelankin R.V., Pronina G.I. Hematological indicators of the marsh frog during stimulated spawning. Timiryazev Biological Journal. 2024;2(2):69-74. (In Russ.) https://doi.org/10.26897/2949-4710-2024-2-2-69-74