Effect of the bioregulators Epin-extra and Novosil on biochemical indicators of oat and barley grain quality

From 2021 to 2022, field experiments with oats and barley were conducted on sod-podzolic soil of average fertility. The experiments involved applying 60 kg/ha of ammonium nitrate, superphosphate, and potassium chloride before sowing the crops. The results showed that treating oat plants with the bioregulator Epin-Extra during the panicle formation phase reduced the synthesis of albumin and easily soluble globulins while increasing the concentration of glutelin proteins during grain maturation. The bioregulator increased the activity of α-amylases and β-amylases at pH 5.5 and 7, as well as of peroxidases at pH 8.0 and catalases at pH 5.5 in the developing grain. The aftereffect of Epin-Extra was observed in germinating oat grain, enhancing the activity of β-amylases at pH 8, as well as all peroxidases and catalases. When applied during the same phase of oat plant development, the bioregulator Novosil initiated the activation of α-amylase synthesis (active at pH 5.5) and β-amylase synthesis (active at pH 8) in ripening grain. It also activated peroxidase (active at pH 7) and catalase (active at pH 5.5) synthesis, as well as α- and β-amylase synthesis (active at pH 8), in grain of 7-day-old seedlings. Treating barley plants with Epin-Extra during the heading phase increased protein synthesis in ripening grain by 0.6-1.2%, as well as the synthesis of amylolytic and antioxidant enzymes. These enzymes include all α- and β-amylases, as well as peroxidases and catalases, which are active at pH 7 and 8. The activation of α-amylases and peroxidases (pH 5.5), some β-amylases (pH 8), and catalases (pH 7 and 8) was observed in germinating grain. The bioregulator Novosil markedly affected protein and enzyme synthesis in ripening barley grain. Under its influence, the activity of some α-amylases and catalases (pH 8), as well as peroxidases and β-amylases (pH 7 and 8), increased. The conducted experiments revealed a more significant impact of the Epin-extra bioregulator on the biochemical processes of ripening and germinating oat and barley grain.

1. Tabur S., Demir K. Role of some growth regulators on cytogenetic activity of barley under salt stress. Journal of Plant Growth Regulation. 2010;60:99-104. https://doi.org/10.1007/s10725-009-9424-6

2. Ponomareva Yu.N., Zaharova O.A. The effect of mineral fertilizers and growth regulator on yield and quality of malt barley when drought. Bulletin of the Ryazan State Agrotechnological University named after P.A. Kostychev. 2015;(3(27)):36-42. (In Russ.)

3. Sorokin A.I., Tsevdenova A.S. Use of growth regulators for spring barley on light-brown soils. Grain Economy of Russia. 2016;(1):35-38. (In Russ.)

4. Sheshegova T.K., Shchennikova I.N. The response of a new barley variety Rodnik Prikamiya to the use of plant growth regulators. Agricultural Science Euro-North-East. 2018;64(3):28-33. (In Russ.) https://doi.org/10.30766/2072-9081.2018.64.3.28-33

5. Evdokimova M.A., Maryina-Chermnykh O.G. Influence of growth regulators on photosynthetic activity of spring barley crops. Vestnik of Ulyanovsk State Agricultural Academy. 2018;4(44):91-97. (In Russ.) https://doi.org/10.18286/1816-4501-2018-4-91-97

6. Watanabe Y., Miura S., Yukawa T., Takenaka S. Effects of plant hormones on Pythium snow rot resistance of barley. Japanese Journal of Crop Science. 2008;1:78-83. https://doi.org/10.1626/jcs.77.78

7. Kozlov F.P., Konova A.M., Samoilov L.N., Ladonin V.F. The effect of chemicals on the yield and quality of crops in field crop rotation. Agrohimia. 2003;(7):24-31. (In Russ.)

8. Gamzaeva R.S. Effect of phytoregulators Epin and Zircon on amylolytic activity and content of reducing sugars in germinating malting barley grains. Izvestiya Saint-Petersburg State Agrarian University. 2016;(44):27-32. (In Russ.)

9. Alam P., Balawi T., Ashraf M., Ahinad P. 24-Epibrassinolide (EBR) reduces oxidative stress damage induced by Cadmium toxicity by restricting uptake and modulating some key antioxidant enzymes in maize plants. Pakistan Journal of Botany. 2021;53(1):59-66. http://dx.doi.org/10.30848/pjb2021-1(27)

10. Persikova T.F., Sergeeva I.I. Application of growth regulators and bacterial preparations on barley and pea crops. Plodorodie. 2006;1(28):19-20. (In Russ.)

11. Novikov N.N., Zharikhina A.A. Protein composition and grain quality of spring soft wheat (Triticum aestivum L.) depending on the level of nitrogen nutrition and phytoregulators use in case of cultivation on sod-podzol medium loamy soil. Izvestiya of Timiryazev Agricultural Academy. 2013;(5):142-152.

12. Shatilova T.I., Karpilenko G.P., Vitol S.B., Shanenko E.F. et al. Effect of metabolic regulators on the protein-proteinase complex of barley grown under different agricultural conditions. Izvestiya of Timiryazev Agricultural Academy. 2005;(3):82-90. (In Russ.)

13. Shatilova T.I., Belopukhov E.V., Romanova Ye.V., Vitol I.S. et al. Amylolytic and proteolytic enzymes of barley malt obtained with the use of phytoregulators. Izvestiya of Timiryazev Agricultural Academy. 2013;(3):31-38. (In Russ.)

14. Vitol I.S., Karpilenko G.P. The protein-proteinase complex of the barley grown on a different agronomic background using regulatory preparations. Applied Biochemistry and Microbiology. 2007;43(3):356-364. (In Russ.)

15. Vitol I.S., Bobkov A.A., Karpilenko G.P. Carbohydrate-amylase complex and technological indicators of the quality of brewing barley grown in the conditions of the Non-Black Earth Region. Izvestiya Vuzov. Food Technology. 2007;2(297):24-27. (In Russ.)

16. Novikov N.N., Solovyeva N.Ye. Formation of brewing properties of barley grain when grown on sod-podzolic soil depending on nutrition pattern and phytoregulator application. Izvestiya of Timiryazev Agricultural Academy. 2020;(2):5-19. (In Russ.) https://doi.org/10.26897/0021-342x-2020-2-5-19

17. Novikov N.N., Tarazanova T.V. Laboratory workshop on plant biochemistry: a study guide. Moscow, Russia: Russian State Agrarian University-Moscow Agricultural Academy named after K.A. Timiryazev, 2012:97. (In Russ.)

18. Novikov N.N. The new method of peroxydases activity determination in plants. Izvestiya of Timiryazev Agricultural Academy. 2016;(3):36-46. (In Russ.)