Analysis of amino acid composition in sheep by-products by High-Performance Liquid Chromatography
https://doi.org/10.26897/2949-4710-2026-4-1-1-02
Abstract
The study aimed to investigate the amino acid composition of sheep by-products (kidney, liver, heart, spleen, blood, and lung) using High-Performance Liquid Chromatography (HPLC). The results showed that 17 amino acids in the samples were successfully detected by HPLC, and the chromatographic peak shape was sharp without interference from extraneous peaks, demonstrating good separation. The amino acid content and composition in by-products serve as key factors in evaluating protein nutritional value and directly influence this value in by-products. Among the six types of samples, there is a significant difference in the content of total amino acids; among them, liver and spleen exhibit the highest total amino acid content, 21.335 g/100 g and 19.325 g/100 g respectively, while blood exhibited the lowest value of 12.49 g/100 g.
About the Authors
Amarzaya Lkhagvasuren
University of Science and Technology
Mongolia
Mongolia
Amarzaya Lkhagvasuren, Research Associate at the Institute of Food Research
Oyunbileg Lkhagvasuren
University of Science and Technology
Oyunbileg Lkhagvasuren, Research Associate at the Institute of Food Research
References
1. Aristoy, M.C. Essential amino acids. In: Nollet L.M.L., Toldra, F. (ed.): Handbook of analysis of edible animal by-products. CRC Press, Boca Raton FL, USA, 2011. pp. 123-135. https://doi.org/10.1201/b10785
2. Honikel, K.O. Composition and calories. In: Nollet L.M.L., Toldra, F. (ed.): Handbook of analysis of edible animal by-products. CRC Press, Boca Raton FL, USA, 2011. pp. 105-121. https://doi.org/10.1201/b10785
3. Anonymous. Animal by-products. Their use in animal nutrition. Feed Compounder 5(10), 1985. 15-16.
4. Lasekan, A., Abu Bakar, F. & Hashim, D. Potential of chicken by-products as sources of useful biological resources. Waste Manage 33(3), 2013. 552-565. https://doi.org/10.1016/j.wasman.2012.08.001
5. Mora, L., Reig, M., Toldra, F. Bioactive peptides generated from meat industry by-products. Food Res. Intern. 2014. 65, 344-349. https://doi.org/10.1016/j.foodres.2014.09.014
6. Martinez-Alvarez, O., Chamorro, S. & Brenes, A. Protein hydrolysates from animal processing by-products as a source of bioactive molecules with interest in animal feeding: A review. Food Res. Intern. 2015. 73, 204-212. http://doi.org/10.1016/j.foodres.2015.04.005
7. Shi, T., Tang, T., Qian, K. and et al. High-performance liquid chromatographic method for determination of amino acids by precolumn derivatization with 4-chloro-3,5-dinitrobenzotrifluoride. Anal Chim Acta. 2009. 654(2): 154-161. https://doi.org/10.1016/j.aca.2009.09.027
8. Deibler, G. E., Martenson, R. E. Determination of methylated basic amino acids with the amino acid analyzer. Application to total acid hydrolyzates of myelin basic proteins. J Biol Chem. 1973. 248(7): 2387-2391. https://doi.org/10.1016/S0021-9258(19)44121-5
9. Xu, W., Zhong, C., Zou, C. and et al. Analytical methods for amino acid determination in organisms. Amino Acids. 2020. 52(12). doi:10.1007/s00726-020-02884-7
10. Gotti, R., Esposito, E., Luise, D. et al. Determination of Free Amino Acids in Milk, Colostrum and Plasma of Swine via Liquid Chromatography with Fluorescence and UV Detection. Molecules. 2022. 27(13): 4153. doi: 10.3390/molecules27134153
11. Qi X. The research of tje effect of leucine supplement in older people muscle protein matabolism. Shanghai: Shanghai Institute of Physical Education, 2015.
12. Holeček, M. Lysine: Sources, Metabolism, Physiological Importance, and Use as a Supplement. International Journal of Molecular Sciences. 2025. 26(18): 8791. doi: 10.3390/ijms26188791
13. Deng, S., Peng, Z., Chen, F. et al. Amino acid composition and anti-anaemia action of hydrolyzed offal protein from Harengula Zunasi Bleeker. Food Chemistry. 2004. 87(1): 97-102. https://doi.org/10.1016/j.foodchem.2003.10.024
14. Juknienė, I., Zaborskienė, G., Jankauskienė, A., et. al. Effect of Lyophilization Process on Nutritional Value of Meat By-Products. Appl. Sci. 2022, 12, 12984. https://doi.org/10.3390/app122412984
15. WHO/FAO/UNU Expert Consultation. PROTEIN AND AMINO ACID REQUIREMENTS IN HUMAN NUTRITION. 2007. WHO Technical Report Series – 935: 265 p. Available online: http://www.fao.org/faostat/en/#data/QL
Review
For citations:
Lkhagvasuren A., Lkhagvasuren O. Analysis of amino acid composition in sheep by-products by High-Performance Liquid Chromatography. Timiryazev Biological Journal. 2026;4(1):102. https://doi.org/10.26897/2949-4710-2026-4-1-1-02
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