Micromorphological studies of skeletal muscle tissue in wild game animals during biological forensics
https://doi.org/10.26897/2949-4710-2025-3-4-1-01
Abstract
This article presents the results of a forensic biological examination of skeletal muscle tissue from wild game animals (maral, elk, reindeer, and Siberian roe deer), conducted using comparative micromorphology within criminal cases concerning illegal hunting (Article 258 of the Criminal Code of the Russian Federation) in the Nazarovsky, Uzhursky, and Uyarsky districts of the Krasnoyarsk Territory. Microstructural studies represent one of the probative methods for species identification of animals, alongside anatomical-topographical, organoleptic, physicochemical, immunological, and genetic research methods. The aim of this study was to identify the microstructural features of skeletal muscle tissues in wild game animals for the purpose of establishing their species and age identification. It was determined that the microstructural features of the gluteus medius muscle tissue in wild game animals include the ratio of muscle to connective tissues, the presence or absence of fat cells in intermuscular spaces, the size of muscle fibers, and the number and area of nuclei per unit area of muscle fiber.
About the Authors
Natalya V. Donkova
Krasnoyarsk State Agricultural University
Russian Federation
Russian Federation
Natalya V. Donkova, DSc (Vet), Professor, Head of the Department of Anatomy, Pathological Anatomy and Surgery, Krasnoyarsk State Agricultural University
Tatyana S. Lebedeva
Russian State Agrarian University – Moscow Timiryazev Agricultural Academy
Russian Federation
Russian Federation
Tatyana S. Lebedeva, СSc (Jur), Associate Professor at the Department of Economic Security and Law, Russian State Agrarian University – Moscow Timiryazev Agricultural Academy
Irina. E. Menchikova
Krasnoyarsk State Agricultural University
Russian Federation
Russian Federation
Irina E. Menchikova, postgraduate student, Assistant at the Department of Anatomy, Pathological Anatomy and Surgery, Krasnoyarsk State Agricultural University
References
1. Criminal Procedure Code of the Russian Federation of 18.12.2001 No. 174-FZ [Electronic Resource] // https://www.consultant.ru/ (date of appeal: 08.10.2025).
2. Resolution of the Plenum of the Supreme Court of the Russian Federation of 18.10.2012 No. 21 "On the Application by the Courts of Legislation on Liability for Violations in the Field of Environmental Protection and Nature Management" [Electronic Resource] // https://www.consultant.ru/ (date of appeal: 08.10.2025).
3. Federal Law of 24.07.2009 No. 209-FZ "On Hunting and Conservation of Hunting Resources and on Amending Certain Legislative Acts of the Russian Federation" [Electronic Resource] // https://www.consultant.ru/ (circulation date: 08.10.2025).
4. Moskalec D.V. Determination of species of wild meat used in the production of canned food by handicraft method. Topical issues and innovative technologies in veterinary medicine, animal husbandry and the environmental complex: materials of the International Scientific and Practical Conference dedicated to the 40th anniversary of the founding of the veterinary faculty. Ussuriysk, November 06-08, 2019. Volume 2. Ussuriysk: Primorsky State Agricultural Academy. 2019:182-189. (Inn Russ.)
5. Ivanchuk G.V., Koval'chuk A.S., Fesechko A.E. Determination of species affiliation of the Himalayan bear at forensic veterinary examination. The role of agrarian science in the development of forestry and agriculture in the Far East: materials of the IV National (All-Russian) scientific and practical conference. In 4 parts, Ussuriysk, November 11-12, 2020/resp. ed. I.N. Kim. - PART 3. Ussuriysk: Primorsky State Agricultural Academy. 2020:279-292. (Inn Russ.)
6. Turdiboev B. M. R. Determination of meat species. Youth and science. 2021;8. (Inn Russ.)
7. Hanhasykov S. P., Zhilin D.N. Forensic veterinary examination of roe deer corpses. Bulletin of the Buryat State Agricultural Academy named after V.R. Filippov. 2023;4(73):169-174. (Inn Russ.)
8. Donkova N.V., Menchikova I.E. Importance of Microstructural Analysis of Skeletal Muscles of Commercial Animals in Forensics. Bulletin of Omsk GAU. 2025;3(59):46-53. (Inn Russ.)
9. Malysheva E.S., Zhukov V.M. Features of microstructural changes in maraluch muscle tissue in the postmortal period. Bulletin of ASAU. 2013;2(100):82-84. (Inn Russ.)
10. Malysheva E.S., Ovcharenko N.D., Mezencev S.V. Assessment of muscular tissue species based on microstructural analysis. Bulletin of ASAU. 2015;4:84-88. (Inn Russ.)
11. Okuskhanova E. K., Asenova B.K., Smol'nikova F.H., Rebezov M.B. Study of the chemical, amino acid composition and microstructure of maral meat of the Bagration peasant farm in the East Kazakhstan region. Agro-industrial complex of Russia. 2021;28(5):671-677. (Inn Russ.)
12. Menchikova I.E., Donkova N.V. Microstructure of some muscles of the Siberian roe deer pelvic limb (Capreolus pygargus). Vestnik KrasSAU. 2024;11(212):162-168. (Inn Russ.)
13. Menchikova I.E., Donkova N.V. Histological and histochemical features of roe deer skeletal muscle. Vestnik KrasSAU. 2025;3(16):187-198. (Inn Russ.)
14. Novak G.V., Bodrova L.F. Histological characteristics of reindeer muscle tissue when using different types of feeding. Bulletin Omsk GAU. 2015;2(52):151-153. (Inn Russ.)
15. Palo Т., Kiessling K.H. Fibre distribution and fibre diameters in two muscles of swedish moose (Alcesalces L.). Comparative Biochemistry and Physiology. Part A: Physiology. 1982;V. 72.(I. 4):669-671.
16. Ruusunen M, Puolanne E. Histochemical properties of fibre types in muscles wild and domestic piqs and the effect of qrowth rate on muscle fibre properties. Meat Science. 2004;67(3):533-539.
17. Zochowska J. et al. Effects of carcass weight and muscle on texture, structure and myofibre characteristics of wild boar meat. Meat science. 2005;Т. 71(№ 2):244-248.
18. Bogucka J., Kapelanski, W., Elminowska-Wenda et al. Comparison of microstructural traits of Musculuslongissimuslumborum in wild boars, domestic pigs and wild boar/domestic pig hybrids. Arch. anim. breed. 2008;№ 51:359-365.
19. Zhenihova N.I., Zashchuk O.N., Kurochkina N.G. Macrocartin and histostructure of yak, beef and moose meat in comparative aspect. Youth and science. 2018;8:84. (Inn Russ.)
20. Bykowska, М. Influence of selected factors on meat quality from farm-raised and wild fallow deer (Dama dama): a review. Canadian Journal of Animal Science. 2018;98(3):405-415. (Inn Russ.)
21. Federal Law on 31.05.2001 No. 73-FZ "On State Judicial Expert Activities in the Russian Federation" [Electronic Resource] // https://www.consultant.ru (circulation date: 08.10.2025).
22. GOST R 58436-2019. National Standard of the Russian Federation "Veterinary Examination of Mechanical Injuries in Unproductive Animals. General requirements," approved by and put into effect by Order of Rosstandart dated 27.06.2019 No. 343-st [Electronic Resource] // https://www.consultant.ru (circulation date: 08.10.2025).
Review
For citations:
Donkova N.V., Lebedeva T.S., Menchikova I.E. Micromorphological studies of skeletal muscle tissue in wild game animals during biological forensics. Timiryazev Biological Journal. 2025;3(4):101. (In Russ.) https://doi.org/10.26897/2949-4710-2025-3-4-1-01
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