In Vitro Callus Induction and Adventitious Shoot Regeneration from Leaf Explants of Heuchera Hybrid
https://doi.org/10.26897/2949-4710-2023-2-28-36
Abstract
The problems of morphogenesis’ induction from isolated leaf explants of the variety Georgia Plum of Heuchera hybrid were studied for the first time. The induction of callus and of adventitious shoots from leaf explants on the modified MS medium containing 30g/l glucose was carried out in the presence of 6-BAP in combination with one of the auxins IAA, IBA, NAA or 2,4-D. The cytokinin: auxin ratio in the medium was 10:1 or 20:1. The best regeneration of Heuchera hybrid (up to 92% of regenerating leaf discs) was obtained on media containing 6-BAP at a concentration of 4.0 mg/l in combination with NAA at a concentration of 0.2 mg/l or 0.4 mg/l. In this case, the number of adventitious shoots per regenerating disc could reach 7‑9 and more. The maximum frequency of direct regeneration was observed on media containing IBA. Abundant callus formation was observed on media containing 2,4-D.
About the Authors
S. A. Muratova
Michurinsk State Agrarian University
Russian Federation
Russian Federation
Svetlana A. Muratova, CSc (Bio), Head of the Biotechnology Teaching and Research Laboratory, Professor of the Department of Horticulture, Biotechnology and Crop Breeding
101, Internatsional’naya Str., Michurinsk, Tambov region, 393760
Yu. V. Khoroshkova
Michurinsk State Agrarian University
Russian Federation
Russian Federation
Yulia V. Khoroshkova, post-graduate student, Department of Horticulture, Biotechnology and Crop Breeding
101, Internatsional’naya Str., Michurinsk, Tambov region, 393760
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Review
For citations:
Muratova S.A., Khoroshkova Yu.V. In Vitro Callus Induction and Adventitious Shoot Regeneration from Leaf Explants of Heuchera Hybrid. Timiryazev Biological Journal. 2023;(2):28-36. (In Russ.) https://doi.org/10.26897/2949-4710-2023-2-28-36
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