Morphological and Genetic Characterization of Cordyceps Militaris Strains in Vietnam
https://doi.org/10.26897/2949-4710-2023-3-78-84
Abstract
Cordyceps (Cordyceps militaris) is a valuable agricultural medicinal crop in many countries. In recent years, this mushroom has become increasingly popular in Vietnam. This study presents the results of a diversity assessment of eight commercial Cordyceps strains collected from different provinces in Vietnam. Morphological characteristics such as color, length, width, and surface features of the mushroom fruiting bodies were studied. Molecular markers of two genes of the MAT1-1 locus (MAT1-1-1, MAT1-1-2) and one gene of the MAT1-2 locus (MAT1-2-1) were used for genetic evaluation. The results of the analysis showed that all the strains were diverse in their morphological characteristics. Seven strains P1, P3, P4, P5, P6, P7, P8 had only one MAT1-1 mating type locus. Only one strain P2 contained two MAT1-1 and MAT1-2 loci simultaneously. This result shows the diversity of Cordyceps strains in Vietnam and supports their selection to create new strains through sexual hybridization.
About the Authors
Ly Nguyen MinhViet Nam
Ly Minh Nguyen, преподаватель, декан of Department of Biotechnology, Faculty of Biology & Environmental Science, Ph.D., кандидат с.-х. наук
459, Ton Duc Thang Str., Da Nang, Vietnam
Suong Le Huynh Thi Ziem
Viet Nam
Suong Le Huynh Thi Ziem, студент факультета биологии и науки об окружающей среде
459, Ton Duc Thang Str., Da Nang, Vietnam
A. A. Mironov
Russian Federation
Aleksey A. Mironov, кандидат с.-х. наук, доцент, доцент кафедры ботаники, селекции и семеноводства садовых растений
49, Timiryazevskaya Str., Moscow, 127434
References
1. Tran T.T., Le V.V. Study of suitable media for the fruiting body of medicinal mushroom Cordyceps militaris on host. Can Tho University Journal of Science. 2020;56(5):125-134. https://doi.org/10.22144/ctu.jvn.2020.121
2. Lee H.H., Kang N., Park I., et al. Characterization of Newly Bred Cordyceps militaris Strains for Higher Production of Cordycepin through HPLC and URP-PCR Analysis. J Microbiol Biotechnol. 2017;27(7):1223-1232. https://doi.org/10.4014/jmb.1701.01043
3. Doyle J.J., Doyle J.L. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull.1987;19(1):11–15.
4. Zheng P. et al. Genome sequence of the insect pathogenic fungus Cordyceps militaris, a valued traditional Chinese medicine. Genome biology. 2012;12:1-22. https://doi.org/10.1186/gb-2011-12-11-r116
5. Lu Y., Xia Y., Luo F., et al. Functional convergence and divergence of mating-type genes fulfilling in Cordyceps militaris. Fungal Genetics and Biology. 2016;88:35-43. https://doi.org/10.1016/j.fgb.2016.01.013
6. Zhang G., Liang Y. Improvement of fruiting body production in Cordyceps militaris by molecular assessment. Archives of microbiology. 2013;195:579-585. https://doi.org/10.1007/s00203-013-0904-8
7. Kang N., Lee H.H., Park I., Seo Y.S. Development of high cordycepin-producing Cordyceps militaris strains. Mycobiology. 2017;45(1):31-38.
8. Lin P.J. et al. Cross breeding of novel Cordyceps militaris strains with high contents of cordycepin and carotenoid by using MAT genes as selectable markers. Scientia Horticulturae. 2021;290:110492. https://doi.org/10.1016/j.scienta.2021.110492
Review
For citations:
Nguyen Minh L., Le Huynh Thi Ziem S., Mironov A.A. Morphological and Genetic Characterization of Cordyceps Militaris Strains in Vietnam. Timiryazev Biological Journal. 2023;1(3):78-84. (In Russ.) https://doi.org/10.26897/2949-4710-2023-3-78-84