Effect of Nitox-trypsin complex hemodynamics and morphobiochemistry in rabbits

Crystalline trypsin possesses anti-inflammatory and anti-edematous properties, allowing it to be used as an alternative to antibiotics. Data on the parenteral use of an enzyme preparation derived from porcine pancreatic glands have shown that it lowers blood pressure in rabbits via a parasympathetic feedback mechanism, consequently exerting a beneficial effect on metabolism. Given the objective of reducing antibiotic usage in animal husbandry, it is necessary to seek ways to create new antimicrobial agents or to lower the dosage of existing preparations. Therefore, hypothesizing that Nitox in complex with trypsin would exhibit synergism, we decided to test the effect of the enzyme preparation in combination with an antibiotic on hemodynamic parameters and the morphobiochemical status of rabbits. Experiments were conducted on 12 Soviet Chinchilla rabbits aged 4.0-6.0 months, with a live weight of at least 3.8 kg. The rabbits in the 1st control group received an intramuscular injection of 0.9% NaCl solution; the 2nd experimental group received Nitox at the recommended dose for rabbits; and the rabbits in the 3rd experimental group received trypsin in complex with Nitox. No changes in hemodynamics were observed in the 1st control and 2nd experimental groups. When using the complex preparation, blood pressure decreased (systolic – by 16.7%, diastolic – by 16.3%, mean – by 16.5%) compared to the background period. The results of biochemical blood tests showed that changes occurred in protein and lipid metabolism in the rabbits, as the experimental group exhibited a decrease in uric acid content by 51.6%, triglycerides by 38.5%, and phosphorus by 18.7% compared to the control group. Hematological results showed that the leukocyte count in the experimental group decreased by 30.8%. Therefore, the complex preparation (Nitox + trypsin) is characterized by a number of unique features compared to Nitox used in its pure form, which provides a basis for testing the preparation in animals for the treatment of inflammatory processes

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