Portable filtration system for chlorophyll determination in expeditionary conditions and cryopreservation of samples
https://doi.org/10.26897/2949-4710-2026-4-1-1-03
Abstract
The study evaluates the effectiveness of two methods for chlorophyll sampling in expeditionary conditions. The first method is based on a portable, syringe-based pressure filtration system, while the second involves cryopreservation of samples. The following were conducted: comparison of syringe-based filtration with conventional vacuum filtration; testing of different filter types (glass-fiber and cellulose acetate); verification of chlorophyll stability in freezing. The results showed that the pressure-driven system with glass-fiber filters provides results comparable to vacuum filtration (5.4 ± 1.52 vs. 6.01 ± 1.15 μg/L); cellulose acetate filters are not recommended for the syringe-based system (underestimation by 55%); cryopreservation preserves chlorophyll for at least 30 days (statistically indistinguishable from freshly collected samples). The proposed modifications of the sampling methods are recommended for widespread use in field conditions.
Keywords
Supporting agencies: The work was carried out within the framework of state assignment No. 125012800888-5
About the Authors
Viktor R. Alekseev
Zoological Institute of the Russian Academy of Sciences
Russian Federation
Russian Federation
Viktor R. Alekseev, DSc (Bio), Professor, Chief Research Associate at the Laboratory of Freshwater and Experimental Hydrobiology
Olga B. Maksimova
Saint Petersburg branch of the Russian Federal Research Institute of Fisheries and Oceanography
Olga B. Maksimova, CSc (Bio), Senior Researcher Associate at the Hydrobiology Laboratory
Olga A. Chaban
Zoological Institute of the Russian Academy of Sciences
Olga A. Chaban, postgraduate student of the Laboratory of Freshwater and Experimental Hydrobiology
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Review
For citations:
Alekseev V.R., Maksimova O.B., Chaban O.A. Portable filtration system for chlorophyll determination in expeditionary conditions and cryopreservation of samples. Timiryazev Biological Journal. 2026;4(1):103. (In Russ.) https://doi.org/10.26897/2949-4710-2026-4-1-1-03
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